The most powerful member of the Image-Pro software family, Image-Pro Plus includes extensive enhancement and measurement tools and allows you to write application-specific macros and plug-ins.

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Acquire Images
Utilize the full precision of your capture equipment with easy-to-use acquisition tools. Image-Pro Plus supports digital cameras, image capture cards, and scanners, disks or CD-ROMs.

• Simple automated controls for complex microscope imagery
• Multi-channel Acquisition for composite images
• Time Lapse Acquisition
• Live Analysis & Measurements

Process and Enhance
Use sophisticated image processing and enhancement tools to uncover details within your images.

• Tile a series of images
• Automatically align either individual or sequence images
• Create a focused image from a series of unfocused images
• Choose from a variety of enhancement and edge filters to filter noise and improve image detail.
• Use a wide range of morphological filters to precisely segment structures and prepare images for automatic measurement.
• Use the Color Composite feature to merge multiple fluorescent images acquired as monochrome single wavelengths into a color composite image.
• Detect co-localization in biological specimens and graphically display the association between the two sets of data in a scatter plot.
• Use pseudo-color to highlight features of interest in a grey scale image. Use colors to visually amplify specific intensities which are normally difficult to distinguish from their surroundings.
• Perform forward and inverse Fast Fourier Transforms (FFTs) to eliminate artefacts

Measure and Classify
Image-Pro Plus includes powerful tools for analysing and obtaining quantitative information from your images.

• Threshold and segmentation allows to extract objects of interest
• Calibration tools can redefine the scale of an image to express values in other units of measure.
• Measure the lengths, areas, perimeters, and angles of objects in your image.
• Determine the thickness of objects by calculating the mean, minimum and maximum thickness between lines.
• Auto or manual trace tools for highlighting features.
• Use the Caliper Tool to detect edges and calculate repeated measurements.
• The colocalization tools provide a visual representation as to the location and degree of overlap between two wavelengths.
• Manually or automatically follow cells or other organisms as they move through time and space using correlation tracking.
  

3D Rendering and Measurement
Explore the depth of your images by visualizing and interacting with them in three dimensions.

• Obtain quantifiable data from your three-dimensional image through manual and volume measurements.
• Make 3D manual measurements of points, lines, angles, circumferences, and enclosing distances
• Accurate 3D volume measurements of surface area, sphericity, feret measurements, bounding box, centroid information and much more.
• Automatic or manual tracking and measuring the movement of individual objects in a 4D sequence using the 4D tracking feature in 3D Constructor.
• Analyse neurons and other filament structures with the neuron tracing tool in 3D Constructor.
• Use colocalization with 3D Constructor to display the colocalized regions in three dimensions.
• Create 3D animations with changing transparencies, slide positions, colors, etc. to give others a better understanding of the depth of your 3D image. Save in AVI or multi-frame TIFF or SEQ formats.

Surface Analysis
Using only the in-focus information derived from a Z-stack of images, 3D Surface Inspector offers unique 3D views and measurements that reveal quality details. With a single camera and Z-motor, you will enjoy this cost-effective and illuminating analysis tool.

• Perform manual measurements such as maximum and minimum Z values over an X/Y line profile, Z height representation over an X/Y line profile, volume covered by that surface area, etc.
• Choose from different depth calculation methods such as controlling Z-motor device directly, collecting Z-stacks directly from Scope-Pro, importing series of Z interval images.
• Control a Prior Scientific Z-motor stage directly from within the application.

Automate, Customize and Share your work
Customization tools and a built-in programming language allow you to streamline your imaging research.

• Automate with easy to create and use Macros.
• Share and download Macros.
• Point out features of interest and add text to your images with Annotation overlay tools.
• Export image data via DDE to Origin® and Microsoft Excel. Create custom reports with images data and text.
• Easily convert sequence files and 3D rendered images to AVI files.
• Customize Image-Pro Plus with the interactive menu editor
• Image-Pro Plus includes a variety of features that allow you alter the application to fit your workflow. Create a personalized Workflow Toolbar with buttons for your most frequently used tools.
  

• Microscope & Stage Automation Included: Image-Pro Plus includes microscope and stage automation tools which were previously available as add-ons. Control your microscope stage in any X, Y, or Z direction. Control motorized filter wheels, shutters, filter sliders, and lamp settings.
• Accelerated Hardware Automation: Automation speeds have been accelerated for the following microscopy hardware controllers:
  • Prior ProScan Controllers
  • Olympus Microscope Stands (BX-61 and IX-81)
  • Sutter Lambda 10-3
  • Ludl MAC
• Just-in-Time Image Loading: Save time by loading your multi-frame image sets on demand, resulting in extremely fast load times. This feature is available for Image-Pro sets and TIFF sequences.
• Optional Live Tiling and Extended Depth of Field: Tile large images and perform Extended Depth of Field operations live – while acquiring your images. This tool is available as an add-on to Image-Pro Plus 7.0.
• Optional 3D Surface Analysis Tools: Use the optional 3D Surface Inspector tool to create beautifully illuminated 3D visualizations of your image stacks. Explore image depth with manual measurements and easily create .avi animations.
• Time-Saving Macros and a Macro Browser: A new Macro Browser (formerly the Macro Player) offers quick access to a variety of timesaving macros. New macros are also available in the Macros Menu.